My code run fast at origin but slowly after!

[AU Gridlock]

Hi, in my code there are many variables declared Private and public
Private lst as list (of string)
Private lst as list (of integer)
Private lst as list (of list (of integer))

.....

I have about 10 such variables (object type) ,it's expensive to load them, so I have them private

, I also put form.hide instead of form.close to load faster.

At first, the code ran smoothly, but I didn't know what the reason was (the number of their elements didn't change but then the code was laggy and slow.
What do I need to do to fix this problem? such as
list = nothing
list = new list (of string)
Does list = nothing work now?
Or I just need
list = new list (of string)
....
Gc.collect ()

Please give me advice!

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